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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Loss of epidermal MCPIP1 is associated with aggressive squamous cell carcinoma

Fig. 5

The expression level of MCPIP1 negatively correlates with the expression levels of the genes encoding SERPINB3/B4 and MMP-9. (a) qRT-PCR analysis of Serpinb3a, -3b, -3c, -3d and Mmp9 expression in papillomas. n = 8-10. (b) qRT-PCR analysis of Serpinb3a and -3b expression levels in whole skin lysates of 3-month-old and 6-month-old mice. n = 3-5. (c) qRT-PCR analysis of SERPINB3, -B4 and MMP9 expression in A431 cells expressing shRNA against MCPIP1. n = 3/5. (d) qRT-PCR analysis of SERPINB3/B4 and MMP9 expression in A431 cells overexpressing MCPIP1 and treated with TPA for 24 h. n = 3. (e) Relative luciferase activity in HEK293 cells transfected with a luciferase reporter plasmid containing the 3’-UTRs of SERPINB3/B4 and MMP9 or the pmirGLO-empty vector together with the control plasmid or the expression plasmid expressing wild-type (WT) MCPIP1 or the D141N mutant. Luciferase activity was normalized to that of the pmirGLO-empty vector. n = 4. The data are shown as the mean ± SD values. Unpaired t-test (A-B) or one-way ANOVA (D-E) was used to calculate P-values. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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