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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Loss of epidermal MCPIP1 is associated with aggressive squamous cell carcinoma

Fig. 6

MCPIP1 modulates the expression of factors related to skin carcinogenesis. (a-b) RNA and protein were isolated from chemically induced papillomas. (a) qRT-PCR analysis of Il6, Il22, Il33, Cxcl2 and Tgfb1 mRNA expression. n = 8-10. (b) ELISA for IL-6, IL-33 and TGF-β1. n = 4. (c) qRT-PCR analysis of IL33 mRNA expression in A431 cells stably expressing shRNAs. n = 5. (d) Relative luciferase activity in HEK293 cells transfected with luciferase reporter plasmids together with the control plasmid or plasmids encoding wild-type (WT) or mutated (D141N) MCPIP1. n = 4. (e) Representative western blot analysis of MCPIP1 protein expression in A431 cells stimulated with TGFβ1. β-Actin was used as the loading control. (f) qRT-PCR analysis of IL6 mRNA expression in A431 cells stimulated with TGFβ1. n = 3. The data are shown as the mean ± SD values. Unpaired t-test (a, b, c, e, f) or one-way ANOVA (d) was used to calculate P-values. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

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