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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: RNA binding protein HuD promotes autophagy and tumor stress survival by suppressing mTORC1 activity and augmenting ARL6IP1 levels

Fig. 4

HuD interacts with and stabilizes GRB-10 and ARL6IP1 RNA transcripts in neuroblastoma cells. A RNA targets of HuD. B HuD-GRB-10 interaction by RIP/RT-qPCR in IMR-32 and HEK293 cells (control vs. FLAG-HuD-overexpressed). C HuD-ARL6IP1 interaction by RIP/RT-qPCR in IMR-32 and HEK293 cells (control vs. FLAG-HuD-overexpressed). D GRB-10, ARL6IP1 and HDAC2 RNA stability assay in IMR-32 and SK-N-SH (comparison of control and silenced HuD). E Western blot analysis of Ago2 and HuD in IMR-32 cells (control or silenced HuD). Full-length blots are presented in Supplementary Fig. S9. F Ago2 bound GRB-10; RIP/RT-qPCR in IMR-32 (control or silenced HuD). G Ago2 occupied ARL6IP1 mRNA by RIP/RT-qPCR in IMR-32 (control or silenced HuD). H Determination of Ago2 occupied GAPDH mRNA (negative control) by RIP assay followed by RT-qPCR in IMR-32 cells (control or silenced HuD). Data are presented as mean ± SEM; t test: **p < 0.01, ***p < 0.001

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