Fig. 4

Expression of OSKM, NKX2-1, SFTPs and DNMT3A/B in A549 and H1299 cells cultured in T-CM and hEML-CM. a mRNA expression levels of OSKM transcription factors in A549 cells cultured in T-CM or hEML-CM. Differences were observed between the cultures for OCT-3/4, KLF4 and c-MYC but not for SOX2. b mRNA expression levels of OSKM transcription factors in H1299 cells cultured in T-CM or hEML-CM. Differences were observed between the cultures for SOX2 and c-MYC. c A549, mRNA expression levels of NKX2-1 and SFTPB. d H1299; mRNA expression levels of NKX2-1 and SFTPD. e A549, mRNA expression levels of DNMT3A and DNMT3B. f H1299, mRNA expression levels of DNMT3A and DNMT3B. Data represent the mean ± SEM from three separate experiments. g, h Phase contrast images of Oil Red O Solution staining of lipid droplets in A549 and H1299 cells lines, respectively, showing positive staining in T-CM and negative staining in hEML-CM. *p < 0.05; **p < 0.01