Fig. 4

CB-839 induces the repression of tongue tumor growth. A The effect of CB-839 on HNSCC cell proliferation after a three-day treatment. B The effect of CB-839 on glucose uptake and lactate release in HNSCC cells. C Changes in secreted glutamate and glutamine consumption were determined by Glutamine/Glutamate-Glo Assays in HNSCC cells with or without CB-839 treatment. D The effect of CB-839 on apoptosis determined by Western blotting. E, F The effect of CB-839 treatment on HN6-derived tongue tumor growth in NSG mice. Representative tongue tumors and quantitative data of tumor volume, tongue weight, and body weight (n = 5/group) are shown in different panels. (G, H) Ki67- and TUNEL-positive tumor cells in tongue xenografts of mice receiving CB-839 or vehicle treatment. Representative images and quantitative data (n = 5) are shown in left and right panels, respectively. Scale bar = 50 μm. *p < 0.05, **p < 0.01