Fig. 6

ACSL4 was targeted by miR-23a-3p. A Predicted miR-23a-3p binding sites in the 3’UTR of ACSL4 mRNA according to the computational algorithms of RNA hybrid. B The mRNA and protein expression of ACSL4 in HCC cell lines after transfection with miR-23a-3p mimics and Anti-miR-23a for 24 h. C ACSL4 expression in the orthotopic HCC tissues showing high ACSL4 in the 23a-KO group. D Luciferase activity of ACSL4 3’UTR after miR-23a-3p mimics transfection in HEK239 cells. E ACSL4 siRNA neutralized the induced accumulation of chelatable iron by Anti-miR-23a upon sorafenib treatment. F and G ACSL4 siRNA inhibited the deposition of lipid peroxides increased by miR-23a-3p inhibitor upon sorafenib treatment. H and I Suppression of cell viability by miR-23a-3p inhibitor under sorafenib treatment reversed by ACSL4 siRNA co-transfection