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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Induction of m6A methylation in adipocyte exosomal LncRNAs mediates myeloma drug resistance

Fig. 2

MM cells exposed to exosomes derived from MM-associated adipocytes are more resistant to chemotherapeutic drugs than those derived from normal adipocytes. a. Schematic for co-cultures of MM cells and adipocytes, and collection of the exosomes from adipocytes. b-d. MM cells were treated with 5 nM bortezomib (BTZ), or 25 μM melphalan (Mel), or 25 nM carfilzomib (CFZ) and with or without adipocyte exosomes for 24 h. Addition of vehicle or PBS served as control for drugs or exosomes, respectively. b. Annexin V-binding assay shows the percentages of apoptotic ARP-1 cells treated with bortezomib and exosomes derived from nADs, PMMADs, and MMADs. c. Annexin V-binding assay shows the percentages of apoptotic primary MM cells, isolated from bone marrow aspirates of patients with newly diagnosed MM, treated with bortezomib and exosomes derived from nADs or MMADs. d. Annexin V-binding assay shows the percentages of apoptotic MM cells treated with various chemotherapeutic drugs, vehicle control, and exosomes derived from nADs or MMADs. Data shown as mean ± SD. ns, non-significant; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. P values were determined by one-way ANOVA

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