Proteins/lipids related to ERRα were enriched in mitochondrial function in EC (A) The relationship between TFEB-ERRα and acc, fasn, acadm are determined by RT-qPCR in KLE and ECC-1 cells. (B) The association of oxygen consumption rates (OCR), basal, maximal respiration, spare respiratory capacity, and ATP production regulated by TFEB-ERRα are shown. (C) The effect of ERRα regulation on TFEB, LPCAT1, LPCAT3, MMP2, and Cortactin expression in EC cells is analyzed using western blot (WB). (D) The effect of XCT790 treatment on LPCAT1, LPCAT3, MMP2, and Cortactin proteins for 24 h were evaluated between control and TFEB-overexpressing by WB. (E) Representative Scanning electron microscope (SEM) micrographs of KLE and ECC-1: TFEB-ERRα axis and XCT790 treatment for 24 h regulated pseudopod, in comparison to the controlled group. Micrographs are screened at scale of 10–30 µM. *, p < 0.05. Statistical tests: Student’ s t-test or ANOVA.