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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: High efficacy and safety of CD38 and BCMA bispecific CAR-T in relapsed or refractory multiple myeloma

Fig. 1

BCMA-CD38 bispecific CAR-T effectively lysed BCMA+CD38+ tumor cells in vitro and in vivo studies. A Diagram of CAR cDNA plasmid and CAR-T cell structures. BCMA-CD38 CAR was composed of BCMA and CD38 targeting domains, human CD8 alpha hinge and transmembrane domain (CD8α hinge + TM), human 4-1BB cytoplasmic domain, and a human CD3 zeta cytoplasmic domain (CD3ζ). BCMA or CD38 CAR was composed of BCMA or CD38 targeting domains, human CD8 alpha hinge and transmembrane domain (CD8α hinge + TM), human 4-1BB cytoplasmic domain, and a human CD3 zeta cytoplasmic domain (CD3ζ). B Representative dot-plots of FCM analysis, detecting the CAR+ expression in T cells 6 days after CD38, BCMA and BCMA-CD38 virus transduction. C Cell indices of BCMA+CD38+ Hela cells were recorded by RTCA after treated with T cells, CD38 CAR-T cells, BCMA CAR-T cells and BCMA-CD38 CAR-T cells at E:T ratios of 0.5 and 2.5. Three replicates were done for each sample. Area under curves (AUC) of cell indices were calculated and statistically analyzed. NS: no statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001. D IL-2, IL-6, IFN-γ and TNF-α levels were compared after treated with T cells, CD38 CAR-T cells, BCMA CAR-T cells and BCMA-CD38 CAR-T cells at E:T ratios of 2.5. NS: no statistically significant, *P < 0.05, **P < 0.01, ***P < 0.001. E Bioluminescence imaging (BLI) was used to assess tumor progression and regression of mice at different time after being treated with T cells and BCMA-CD38 CAR-T cells. Quantification of BLI as curves was presented. **P < 0.01, ***P < 0.001. F Representative dot-plots of FCM analysis, detecting the percentage of tumor cells in mice treated with T cells and BCMA-CD38 CAR transducing T cells on day 36. The cells were stained with GFP and anti-Human CD45. The blue populations were CAR-T cells expressing human CD45, and the red populations were MM cells expressing GFP and human CD45

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