Fig. 2

INPP5F promotes HCC cell proliferation in vitro. A QRT-PCR and (B) western blot confirmed the efficiencies of INPP5F knockdown in SK-Hep1 as well as MHCC-97H and overexpression in Huh7, respectively. C The effect of INPP5F on cell proliferation was determined by EdU assays. The representative images and the percentage of EdU positive cells are shown. D Colony formation was performed to validate the impact of INPP5F on cell proliferation. E The cell cycle analysis was performed in cells with INPP5F knockdown or overexpression. The percentage of cells in G1, S and G2 phase was indicated. Data is presented as means ± standard error for three independent experiments, *P < 0.05, ***P < 0.001