Fig. 6

INPP5F translocates into cytoplasm to exhibit its oncogenic activity. A INPP5F was commonly nuclear staining in cells of adjacent non-tumor tissues, and cytoplasmic staining in HCC cells. B-D Indicated cells were treated with LMB (SK-Hep1: 10 ng/ml and MHCC 97H: 40 ng/ml) for 6 h, and then were subjected to (B) immunofluorescence for detection of sub-cellular localization of INPP5F, (C) western blot for detection of INPP5F protein expression in nuclear and cytoplasm. D western blot for detection of the expression of INPP5F-related downstream molecules. E LMB-induced nuclear restriction of INPP5F affected cell proliferation. Scale bar: 100 um