Fig. 4

Bufalin decreases cellular proliferation and PI resistance induced by AHSA1/HSP90 in MM cells. A-B Effects of Bufalin (60nM) and BTZ (10nM) incubation for 48h on cell apoptosis of ARP1 (A) and H929 (B) WT and AHSA1-OE cells. C The rate of drug-induced apoptosis was shown in the histogram. D Effects of Bufalin on the expression of CDK6 and PSMD2 in ARP1 and H929 WT and AHSA1-OE cells. E-F Co-IP assay revealed that Bufalin interfered the interaction between HSP90 and AHSA1 in ARP1 and H929 cells. G Co-IP assay confirmed the interaction between AHSA1, HSP90, CDK6, PSMD2 and the activated form of CDK6, phosphorylation of Y13 site at CDK6. H-J Proteasome activity assay verified that Bufalin inhibited proteasome activity in (H) ANBL6 WT/DR cells, I ARP1 and H929 AHSA1 WT/OE cells and J PSMD2 WT/OE cells. The data are expressed as mean ± SD.*p<0.05, **p<0.01, ***p<0.001