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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: cPLA2 blockade attenuates S100A7-mediated breast tumorigenicity by inhibiting the immunosuppressive tumor microenvironment

Fig. 6

cPLA2 inhibitor increases the infiltration of proliferative activated CD4/CD8 tumor-infiltrating T lymphocytes (TILs) in TME of tumor bearing S100A7 overexpressing mice (A). Schematic diagram of CODEX workflow showing the different steps of FFPE TMAs staining (B). Seven-color image of FFPE TMA prepared from of orthotopic syngeneic breast cancer model of mS100a7a15 bi-transgenic mouse model and imaged using a 25 DNA-barcode conjugated antibodies panel. 7 colors show the following markers: red- CD8a, yellow- CD45R, cyan- CD11b, White- CD90.2, blue- CD4, magenta- CD38, and green- Ki67 (C). Heatmap showing the average strength of cell-type to cell-type interaction for different clusters of vehicle control (VC) and cPLA2 inhibitor (AF3) treated mS100a7a15 overexpression mouse model (D). t-SNE and heatmap showing the expression of different markers associated with proliferating and activated CD4+ and CD8+ T cells (E). Circos plot and, (F). heat map showing the cell interaction networks of different subsets of proliferating and activated CD4+ and CD8+ T cells in VC and AF3 treated groups. The thickness of interacting connection associates with the number of contacts, size of the node represents the number of cells per group. Scale bar in heat map showing the interaction strength of different connecting nodes (G). Spearman rho’s correlation coefficient analysis of cPLA2 mRNA (PLA2G4A) expression with infiltration level of CD4 and CD8 T cells in invasive basal-like breast cancer patients (source: TIMER2.0; http://timer.cistrome.org/)

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