Fig. 7
cPLA2 inhibition obstructs tumor growth and metastasis in Hu-PDX mice model by attenuating immunosuppressive cells (A). Immunoblot analysis of S100A7 and cPLA2 proteins in tumor lysate of breast cancer patient derived-xenograft (PDX) specimen. GAPDH was used as a loading control (B). Schematic diagram showing the methodology for generation and treatment strategy of humanized PDX (Hu-PDX) breast cancer model (C). Representative image of tumors harvested from VC or AF3 (5 mg/kg.bt) treated Hu-PDX mice model. Graph showing the (D). tumor volume (mm3) and (E). Tumor weight (gm) of VC or AF3 treated PDX mice model (F). H/E image of lung nodules in lungs harvested from VC or AF3 treated Hu-PDX groups. The graphs indicate the means ±SEMs of four replicates. Flow cytometric analysis of % (G). total CD68+ TAMs (out of EpCAM−), (H). CD163+M2-TAMs (out of EpCAM−CD68+ macrophages) and (I). Exhausted CTLA+ CD4 T (out of EpCAM−CD14−CD3+CD4+) cells (J). PD-L1+ EpCAM+ tumor cells harvested from VC or AF3 treated Hu-PDX groups. The graphs indicate the means ±SEMs of three replicates. ns: non-significant, *P < 0.05, ** P < 0.01, *** P < 0.001. t test was used for statistical significance