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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: M1-like tumor-associated macrophages cascade a mesenchymal/stem-like phenotype of oral squamous cell carcinoma via the IL6/Stat3/THBS1 feedback loop

Fig. 3

Significant correlations between M1-like TAMs and EMT/CSCs status of OSCC cells indicated by mRNA sequencing and further validation. A. Volcanic plots for the mRNA sequencing of SCC25 cells and Cal27 cells treated by CM from M1-like TAMs or M0 cells; B. Venn diagram indicating 148 differential expression genes shared by SCC25 cells and Cal27 cells; C. GO analysis for the up-regulated differential expression genes shared by SCC25 cells and Cal27 cells; D. Heat-map from the UCSC Xena Browser based on the TCGA primary HNSCC cohort depicted the gene expression relationship between M1 related markers (CD68, CD80, and CD86) and EMT related markers (VIM, SNAI1, TWIST1, and CDH2). E. Heat-map from the UCSC Xena Browser based on the TCGA primary HNSCC cohort depicted the gene expression relationship between M1 related markers (CD68, CD80, and CD86) and CSC related markers (MET, MME, and MMP14); F-G. Significantly increased expression of EMT related markers (VIM and CDH2) in OSCC cells treated by CM from M1-like TAMs for 4 days; H-I. Significantly increased expression of CSC related markers (MME and MMP14) in OSCC cells treated by CM from M1-like TAMs for 6 days; J. An increased expression of N-cadherin (encoded by CDH2), Vimentin (encoded by VIM), CD10 (encoded by MME), and MT1-MMP (encoded by MMP14) in OSCC cells treated by CM from M1-like TAMs for 7 days; K-M. Representative images and relative expression for the Ki67 (K), N-cadherin (L), and Vimentin (M) of the xenografts (200x); **p<0.01

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