Fig. 4

IL6 secreted from the M1-like TAMs activated by exosomes from OSCC cell lines. A. Cytokine expression profiles assessed by Luminex assay. THP-1 and PMNCs derived macrophages were treated with exosome supernatant from SCC25 and Cal27 for 24h, respectively; B. Validation for the mRNA expression of IL6 in the THP-1 and PMNCs derived macrophages treated with exosome supernatant from SCC25 and Cal27 for 12h; C. Validation for the IL6 secretion in the THP-1 and PMNCs derived macrophages treated with exosome supernatant from SCC25 and Cal27 for 24h; Data were represented as the mean ± SD of three independent experiments, **p<0.01. D. Heat-map from the UCSC Xena Browser based on the TCGA primary HNSCC cohort depicted the gene expression relationship between IL6 and CD68, CD80 or CD86; E. Expression pattern of IL6, CD68, CD86, and CD80 in a validated primary OSCC cohort (n=25). Crosstab analyses were performed for all the involved cases and Chi-squared tests were performed to assess the expression correlations between two variables **p<0.01