Fig. 3

Modulation of fatty-acid-synthesis related genes by globular adiponectin in breast cancer cells. MCF-7 (A, C, E, and H) and MDA-MB-231 (B, D, F, G, and I) cells were treated with gAcrp (1 μg/mL) for indicated time periods. A-B The expression levels of precursor and nuclear SREBP-1 were determined by western blot analysis. C-D SREBP-1 mRNA levels were measured by RT-qPCR. E-F The SREBP-1 specific dsDNA binding activity was examined as described in Methods. G The expression of the genes modulating fatty acid synthesis, including FASN, ACC-1, ACLY, FADS1, FADS2, and SCD-1, were analyzed by RT-qPCR. H-I The protein levels of FASN were examined by western blot analysis. * indicates p < 0.05 compared to control; n=3 except where specifically indicated in Figures