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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Adiponectin triggers breast cancer cell death via fatty acid metabolic reprogramming

Fig. 6

Involvement of SIRT-1 induction and mTOR signaling in the modulation of SREBP-1 by globular adiponectin. A-D MCF-7 cells were treated with gAcrp (1 μg/mL) for 8 h (A-B) or transfected with 25 nM SIRT-1 siRNA for 36 h before treatment with gAcrp for 8 h. (A and C) Acetylated SREBP-1 level was determined by immunoprecipitation with an anti-acetyl lysine antibody and western blot analysis using an anti-SREBP-1 primary antibody. B and D Ubiquitinated SREBP-1 level was measured by immunoprecipitation with an anti-ubiquitin antibody, followed by western blot analysis using an anti-SREBP-1 primary antibody. E-F Cells were treated with gAcrp (1 μg/mL) for different time periods. The total and phospho-mTOR levels were determined by immunoblotting. G-H MCF-7 cells were transfected with SIRT-1 siRNA for 36 h (G) or pretreated with EX527 for 2 h (H), followed by treatment with gAcrp for additional 3 h. p-mTOR/mTOR levels were examined by western blot analysis. I-J MCF-7 cells were treated with different concentrations of rapamycin (I) and MHY1485 (J) for 8 h. SREBP-1 and FASN expression levels were measured by western blot analysis. K-L MCF-7 cells were treated with rapamycin and MHY1485 for 24 h. Intracellular lipid accumulation (K) and free fatty acid (L) levels were determined as described above. * indicates p < 0.05 compared to control; # indicates p < 0.05 compared to gAcrp-treated cells; n=3

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