Fig. 6

ZFP64 induces immune microenvironment changes. A-B CyTOF analysis and quantification of immune cell populations was performed in humanized NSG mice with different ZFP64 expression. C-D T cells were cocultured with HGC-27 cells, and the apoptosis rate of T cells was assayed using Annexin V/PI staining in combination with FACS analysis. E An inflammatory cytokine antibody array was used to assess cytokine secretion from HGC-27/MGC-803-ZFP64 cells, control cells and blood lymphocyte coculture supernatants. The left panel shows the cytokine antibody array incubated with the supernatant of HGC-27-ZFP64/control cells and blood lymphocyte coculture. Data are presented as the gray ratio between two groups. F Representative images of immunohistochemical staining for ZFP64, GAL-1 and CD8 in the TMA of gastric cancer. G Pearson’s correlation analysis of the expression of ZFP64 and CD8 in TMA (upper panel) and ZFP64 and GAL1 expression in TMA (lower panel). H-I The increase in T cell apoptosis induced by ZFP64 overexpression in GC cells was abrogated by GAL-1 knockdown. J-K An inflammatory cytokine antibody array was used to assess the changes in cytokine levels between HGC-27/MGC-803-ZFP64-shGAL-1 cells and their control cells cocultured with blood lymphocytes. Data are presented as the means±SEM. * P < 0.05; ** P < 0.01