Fig. 1

HDAC3 and TGIF1 are highly expressed and miR-296-3p is lowly expressed in CRC tissues and cells. a-c. RT-qPCR detection of HDAC3, miR-296-3p and TGIF1 expression in CRC tissues and adjacent normal tissues; d-f. Pearson correlation analysis of the correlations among HDAC3 mRNA, miR-296-3p and TGIF1 mRNA expression in CRC tissues; g. Protein bands of HDAC3 and TGIF1 protein in CRC tissues and adjacent normal tissues; h. Comparisons of HDAC3 and TGIF1 protein expression in CRC tissues and adjacent normal tissues; i & j. Representative tissues immunohistochemically stained for HDAC3 in CRC tissues and adjacent normal tissues (× 400) and quantitative analysis of immunohistochemistry of HDAC3; k. RT-qPCR detection of HDAC3, miR-296-3p and TGIF1 expression in FHC and CRC cell lines; l. Protein bands of HDAC3 and TGIF1 protein in FHC and CRC cell lines; m. Comparisons of HDAC3 and TGIF1 protein expression in FHC and CRC cell lines; n-p. Pearson correlation analysis of the correlation of HDAC3 mRNA, miR-296-3p and TGIF1 mRNA expression in CRC cells. In Fig. a-j, n = 121; In Fig. k-p, N = 3. Comparisons between two groups were evaluated by t test while those among multiple groups by one-way ANOVA, followed by Tukey’s multiple comparisons test. The correlations among HDAC3 mRNA, miR-296-3p and TGIF1 mRNA expression in CRC tissues and cells were analyzed by Pearson correlation analysis. * represented P < 0.05, ** represented P < 0.01, *** represented P < 0.001