Fig. 2

circCUL2 is critical to maintain CAFs pro-tumor activity in vitro. A-B EdU assay of the proliferation of PANC-1 cells treated with conditioned medium from circCUL2-overexpression NFs or circCUL2-silencing CAFs. Scale bar: 100 μm. C-D Colony formation assays in PANC-1 cells treated with conditioned medium circCUL2-overexpression NFs or circCUL2-silencing CAFs. E-F Scratch wound healing assays in PANC-1 cells treated with conditioned medium circCUL2-overexpression NFs or circCUL2-silencing CAFs. Scale bar: 100 μm. G-H Transwell assays of migration and invasion of PANC-1 cells treated with conditioned medium circCUL2-overexpression NFs or circCUL2-silencing CAFs. Scale bar: 100 μm. Data are expressed as the mean ± SD of three independent experiments. ^**p < 0.01 and ^***p < 0.001 (two-tailed Student t-tests)