Fig. 2

Expression of FOXQ1 in CRC tissues and cells and its correlation with the prognosis of CRC patients. A qRT-PCR was used to determine the mRNA expression of FOXQ1 in clinically collected CRC tissues and adjacent normal tissues (n = 83, * p < 0.05 compared with adjacent normal tissues); B IHC was used to determine the protein expression of FOXQ1 in clinically collected CRC tissues and adjacent normal tissues (n = 83, * p < 0.05 compared with adjacent normal tissues); C Kaplan-Meier method was used to analyze the correlation between FOXQ1 protein expression and the overall prognostic survival of CRC patients (n = 83); D qRT-PCR was used to determine the mRNA expression of FOXQ1 in the normal human colonic epithelial cell line NCM460 and 4 CRC cell lines (* p < 0.05 compared with NCM460 cells); E Western blot assay was used to measure the protein expression of FOXQ1 in the normal human colonic epithelial cell line NCM460 and 4 CRC cell lines (* p < 0.05 compared with NCM460 cells). All cell experiments were repeated for three times independently. The measurement data were expressed as mean ± standard deviation. Unpaired t test was adopted for comparison between groups, one-way ANOVA was used for comparison among multiple groups, and Tukey’s test was selected for pairwise comparison within the group. The survival curve was tested by Log-rank method.