Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: FOXQ1-mediated SIRT1 upregulation enhances stemness and radio-resistance of colorectal cancer cells and restores intestinal microbiota function by promoting β-catenin nuclear translocation

Fig. 5

Impact of FOXQ1 on SIRT1 transcriptional expression in CRC. A qRT-PCR was used to determine the mRNA expression of SIRT1 in CRC tissues and adjacent normal tissues (n = 83; * p < 0.05 versus adjacent normal tissues); B IHC was used to determine the protein expression of SIRT1 in CRC tissues and adjacent normal tissues (n = 83; * p < 0.05 versus adjacent normal tissues); C qRT-PCR was used to determine the mRNA expression of SIRT1 in the normal colonic human epithelial cell line NCM460 and 4 CRC cell lines (* p < 0.05 between two groups); D Pearson correlation analysis of SIRT1 and FOXQ1 expression (n = 83); E ChIP assay was carried out to test the enrichment of FOXQ1 in SIRT1 promoter region in CRC tissues and adjacent normal tissues (n = 83; * p < 0.05 versus adjacent normal tissues); F qRT-PCR was used to measure the SIRT1 mRNA expression in HCT116R and HT29R cells in response to FOXQ1 knockdown/overexpression (* p < 0.05 between two groups); G Western blot assay was carried out to measure the SIRT1 protein expression in HCT116R and HT29R cells in response to FOXQ1 knockdown/overexpression (* p < 0.05 between two groups); H The dual-luciferase reporter assay verified the binding of FOXQ1 to SIRT1 promoter region in HCT116R and HT29R cells (* p < 0.05 versu sh-NC-treated cells); I ChIP assay was carried out to detect the enrichment of FOXQ1 in SIRT1 promoter region after knocking down or overexpressing FOXQ1 in HCT116 and HT29R cells (* p < 0.05 verusu cells treated with sh-NC or oe-NC); * p < 0.05 in comparison between two groups, and # p > 0.05 compared with HT29 cells. All cell experiments were repeated for three times independently. The measurement data were expressed as mean ± standard deviation. Unpaired t test was adopted for the comparison between two groups. The correlation analysis was carried out by Pearson method

Back to article page