Fig. 7

Impact of SIRT1 on nuclear translocation and activity of β-catenin in CRC. A qRT-PCR was used to determine the mRNA expression of β-catenin in CRC tissues and adjacent normal tissues (n = 83, * p < 0.05 compared with adjacent normal tissues); B IHC was used to measure the protein expression positive rate of β-catenin in CRC tissues and adjacent normal tissues (n = 83, * p < 0.05 compared with adjacent normal tissues); C qRT-PCR was used to determine the mRNA expression of β-catenin in the normal human colonic epithelial cell line NCM460 and 4 CRC cell lines; D Pearson correlation analysis was used to evaluate the correlation between β-catenin and expression of SIRT1 (n = 83); E qRT-PCR was used to determine the mRNA expression of SIRT1 and β-catenin in HCT116R and HT29R cells in response to SIRT1 overexpression/inhibition (* p < 0.05 compared with DMSO or oe-NC); F IP assay was carried out to detect the acetylation level of β-catenin in HCT116R and HT29R cells in response to SIRT1 overexpression/inhibition; G Western blot assay was used to measure the protein expression of p-β-catenin and Cyclin D1, a downstream target gene of β-catenin, in HCT116R and HT29R cells in response to SIRT1 overexpression/inhibition (p < 0.05 verusu cells treated with DMSO or oe-NC); H TOP/FOP luciferase reporter was used to detect the luciferase activity of β-catenin in HCT116R and HT29R cells in response to SIRT1 overexpression/inhibition; I immunofluorescence was used to detect the nuclear translocation of β-catenin protein in HCT116R and HT29R cells in response to SIRT1 overexpression/inhibition (p < 0.05 between two groups). All cell experiments were repeated for three times independently. The measurement data were expressed as mean ± standard deviation. Unpaired t test was adopted for the comparison between groups. Correlation analysis was carried out by Pearson. One-way ANOVA was used for multi-group comparison, and Tukey’s test was selected for pairwise comparison within the group