Fig. 9
Impact of FOXQ1/SIRT1/β-catenin axis on xenograft formation ability within radiation-resistant CRC cells. A qRT-PCR was used to determine the mRNA expression of FOXQ1, SIRT1 and β-catenin in the xenograft tissues of nude mice; B IHC was used to measure the protein expression positive rate of β-catenin in the xenograft tissues of nude mice; C qRT-PCR measurement of the levels of Gammaproteobacteria, Enterobacteriaceae and Fusobacteriales in the feces of nude mice; D qRT-PCR was used to determine the mRNA expression of tumor stem cell markers (CD133, SOX2 and OCT4) in the xenograft tissues of nude mice; E Western blot assay was used to determine the protein expression of Cyclin D1 in the xenograft tissues of nude mice; F Representative tumor anatomy of nude mice; G Broken line graph of volume growth change in the xenograft of nude mice; H Weight comparison of xenograft of nude mice; * p < 0.05 compared with sh-NC + oe-NC group, # p < 0.05 compared with sh-NC + oe-β-catenin group. Each group consists of 8 nude mice. The measurement data were expressed as mean ± standard deviation. One-way or repeated measurement ANOVA was used for multi-group comparison, and Tukey’s test was selected for pairwise comparison within the group