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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Genome-wide CRISPR/Cas9 library screen identifies PCMT1 as a critical driver of ovarian cancer metastasis

Fig. 6

PCMT1 regulates the integrin-FAK-Src pathway through LAMB3. A and B Western blot analysis of the phosphorylation of FAK-Src and AKT in PCMT1-OE SKOV3 cells (A) and PCMT1-KO SKOV3 cells (B). C The impact of rescue expression of PCMT1 on FAK-Src signaling in PCMT1-KO SKOV3 cells was also determined. D PCMT1-KO cells were incubated with the supernatant (SN) from PCMT1-OE SKOV3 cells, and FAK-Src signaling was determined. E The effect of SN incubated with PCMT1 blocking antibody on FAK-Src was also examined under the same conditions. F–H The effects of FAK silencing on cell migration (F) and living cell spheroid formation (G) along with FAK-Src phosphorylation (H) were detected in PCMT1-OE SKOV3 cells. I After silencing LAMB3 in PCMT1-OE SKOV3 cells, FAK-Src signal activity was determined. J Control (sgControl) and PCMT1-KO (sgPCMT1) SKOV3 cells were transfected with EGFP-tagged paxillin, and cell FA dynamics were monitored and photographed every 5 min. Representative frames from time-lapse sequences (left) and quantification of duration time are shown (right, n = 20). (scale bar: 15 μm; Data are shown as mean ± SEM of 3 independent experiments. Data were statistically analyzed with Student's t-test. *P < 0.05; **P < 0.01; ***P < 0.001.)

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