Fig. 4
From: RelB upregulates PD-L1 and exacerbates prostate cancer immune evasion
Silencing RelB in PC-3 cells enhances the immunities of CD4+ and CD8+ T cells. a-b PC-3 cells were cocultured with activated T cells derived from human PBMCs. CD4+ and CD8+ T cells were quantified by flow cytometry using the relevant antibodies. c-d Subsequently, the proliferation of CD4+ and CD8+ T cells was analysed by flow cytometry. The percentage of each generation (cell division) was plotted. e After coculture with activated T cells, the survival rate of PC-3 cells was measured using a clonogenic assay. *(p < 0.05) and **(p < 0.01) show significance between the two groups as indicated