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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: RelB upregulates PD-L1 and exacerbates prostate cancer immune evasion

Fig. 6

RelB deprivation reduces mouse xenograft tumour growth. a RM-1 (control), RM-1:RelB-KO and RM-1:RelB-KO/PD-L1 cell lines were cultured and then subcutaneously injected into male C57BL/6 mice for tumour formation. After tumours reached the maximal volume, the mice were sacrificed and the excised tumour tissues were photographed. b Tumour volume was measured every other day, and the tumour growth rate was determined as plotted in each group. *(p < 0.05), **(p < 0.01) show the significance in RelB-KO cells and RelB-KO/PD-L1 compared to the control cells; and #(p < 0.05), ##(p < 0.01) indicate the significances in RelB-KO/PD-L1 cell vs. RelB-KO cells. c The expression levels of RelB and PD-L1 in the tumour tissues were measured by immunoblots. d-e The levels of RelB, PD-L1, CD4 and CD8 proteins in the tumour tissues were quantified by IHC and the relative H-scores were plotted. *(p < 0.05), **(p < 0.01) show significance between the two groups as indicated. f Blood samples were collected from mice on different days after cell injection as indicated to analyse the percentages of CD4+ and CD8+ T cells by flow cytometry. *(p < 0.05), **(p < 0.01) show significance between the two groups as indicated

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