Fig. 8

Exo-miR-155-5p activates CD8 positive T cells in vivo. A. Exosomes in ascites and serum were isolated and purified. The expression of exo-miR-155-5p was determined by RT-qPCR (n = 4–7). B. The expression of macrophage marker F4/80 was analyzed by IHC in tumor tissues. Representative images of HE staining and IHC are shown (n = 5). Scale bar = 200 μm. C. The proportions of macrophages in tumors were measured by cell sorting (n = 3–4). D. Macrophages were isolated from ascites and spleen using FACS followed by miR-155-5p detection with RT-qPCR (n = 3–6). E. Flow cytometry was performed to determine the PD-L1 expression in macrophages from ascites (n = 6), spleen (n = 4–5), and tumor tissues (n = 4–5). Mean Fluorescence Intensity (MFI) was statistically analyzed. F. The PD-L1 expressions were analyzed in tumor tissues by immunoblotting (n = 4/group). The densitometry of bands was quantified with image J. G. The expression PD-L1 in tumor tissue was determined by IHC. Representative images IHC are shown (n = 5). Scale bar = 200 μm. H. CD3⁺ CD8⁺ T cell subsets in ascites and spleen were analyzed by flow cytometry. The percentages of CD8⁺ cells were indicated (n = 6–7). Data = Mean ± SD. *p < 0.05, **p < 0.01