Fig. 1

NUP98-NSD1 interacts with SMARCA5 and its cancer co-dependent genes BPTF, and NUP188. A Volcano plot showing the enrichment of the bait FLAG-NUP98-NSD1 and the top 20 most enriched interactors (red dots). B Western blot validation of NUP98-NSD1 interactions. C Cancer dependency analysis of top 20 most enriched interactors. The proteins whose expression is essential for the growth of hematopoietic, blood or acute myeloid leukemia cell lines (KDM1A, SMARCA5, WDR18, ZNF198, TRIM28) are marked in light red. According to Depmap.org, SMARCA5 exhibits co-dependency with BPTF and NUP188 in cancer, while other blood-related top20 interactors do not have cancer-related partners within the core-NUP98-NSD1 interactome. D Venn diagram shows the overlap between NUP98-NSD1 and SMARCA5 gene targets. The plot indicates the most represented biological processes belonging to common gene targets