Fig. 2
From: SMARCA5 interacts with NUP98-NSD1 oncofusion protein and sustains hematopoietic cells transformation
NUP98-NSD1 interactome binding relies on disordered FG repeat domains. A Scheme displaying deletion of specific NUP98-NSD1 domains. B Principal component analysis assessing the level of variation between the interactor abundances (LFQ values) within the full-length and mutated fusion interactomes. C Heatmap shows the log2 fold change (log2 transformed ratio between the LFQ values of the interactors bound by NUP98-NSD1 vs the mutated constructs) of top20 most confident NUP98-NSD1 interactors upon specific domain deletions in NUP98-NSD1. D Co-immunoprecipitation coupled to Western blot validation of lost interactions upon deletion of single or both FG repeat domains