Fig. 2

PAPTP-induced pathological CLL cell death in Eμ-TCL1 mice. a Scheme representing the adopted protocol for PAPTP treatment: mice were injected once a day for 5 consecutive days; after 2 days of recovery, mice were subjected again to 5 injections, once a day for 5 consecutive days. b-d The percentage of B (CD19⁺CD5⁺) CLL cells was assessed by flow cytometry on ex vivo peripheral blood cells from Eμ-TCL1 mice before and after intraperitoneal administration of DMSO or 5 nmol/g PAPTP. Data were obtained from 15 independent experiments for each condition. Statistical significance of differences was assessed with the Mann–Whitney test. Average differences between the post- and pre-treatment percentages of CD19⁺CD5⁺ cells are reported for each substance (b), as well as individual percentages before and after treatment (c-d). Statistical significance of differences was assessed with the Mann–Whitney test. e-g Percentages of CD19⁺CD5⁺ cells were assessed by flow cytometry on ex vivo cells from the peritoneal cavity (e), spleen (f), and bone marrow (g) of Eμ-TCL1 mice after i.p. administration of DMSO (or PAPTP)