Fig. 3

PAPTP reduces the absolute number of pathological CLL cell death in different body districts Eμ-TCL1 mice. a The absolute number of B (CD19⁺CD5⁺) CLL cells was assessed by flow cytometry on ex vivo cells from peripheral blood from Eμ-TCL1 mice after intraperitoneal administration of 5 nmol/g PAPTP as in Fig. 2 (n = 7). Statistical significance of differences were assessed with the Wilcoxon test. b The absolute number of B (CD19⁺CD5⁺) CLL cells was assessed by flow cytometry on ex vivo cells from peritoneal cavity and spleen from Eμ-TCL1 mice after intraperitoneal administration of DMSO. Please note the logarithmic scale in y axis. Each mouse is indicated with a different color (n = 4). On the right, images of the spleens of the four examined animals are shown. In c leukemia burden is estimated by the sum of the absolute numbers of CLL B cells from peritoneal cavity and spleen in 8 animals (n = 4 for DMSO treatment, n = 4 for PAPTP treatment, as in Fig. 2a). Statistical significance of differences was assessed with the Mann–Whitney test. d as in c) for bone marrow is shown. e Percentages of healthy (CD19⁺CD5⁻) and CLL (CD19⁺CD5⁺) B cells, as well as of T (CD19⁻CD5⁺) cells, were assessed by flow cytometry on ex vivo PB cells from Eμ-TCL1 mice before and after i.p. administration of DMSO or 5 nmol/g PAPTP. Mice were assigned to each group on the basis of the percentage of CD19⁺CD5⁺ cells, to minimize the difference in the distribution between the two groups; the absence of significant differences between the other cellular subtypes was assessed with the Mann–Whitney test. The relative abundance of each cellular subtype on the total lymphocytic number is reported for each condition. Data were obtained from 11 independent experiments, for each condition, as reported in Fig. 2b-d. Statistical significance of differences was assessed with the Mann–Whitney test