Fig. 4

PYCR1 interference reduces proliferation in vitro. OPM-2 and RPMI-8226 were cultured in a hypoxic environment and knockdown was established using 20 nM siPYCR1 and/or 50 nM siPYCR2. A, B Relative BrdU incorporation was measured by BrdU-FITC positivity on flow cytometry after 72 h (OPM-2) and 48 h of culture (RPMI-8226) (n = 5). C) Survival and proliferation markers were measured on protein level by western blotting after 72 h of culture (n = 5). D Bar graphs represent the pixel intensity measured by Image Studio Lite v5.2. All graphs are shown relative to mock (n = 5). All experiments were performed in hypoxic conditions. ND = not detected. Significance was determined by one-way ANOVA. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001