Fig. 5

PYCR1 interference inhibits protein synthesis by downregulating the PRAS40 pathway. OPM-2 and RPMI-8226 were incubated with 20 nM siPYCR1 and cultured in a hypoxic environment. A, B Western blot analysis of protein synthesis-related proteins, isolated after 72 h (or 96 h in case of OPM-2 p-eIF4E, eIF4E, p-4EBP1, 4EBP1) of culture. Bar graphs represent the pixel intensity measured by Image Studio Lite v5.2. All graphs are shown relative to mock (n ≤ 5). C Overview of the PRAS40 pathway. Created with BioRender.com. D, E Western blot analysis of puromycin uptake 72 h after PYCR1 knockdown is initiated (n = 5). All experiments were performed in hypoxic conditions. Significance was determined by Kruskal–Wallis or one-way ANOVA * p ≤ 0.05, ** p ≤ 0.01