Skip to main content
Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Pyrroline-5-Carboxylate Reductase 1: a novel target for sensitizing multiple myeloma cells to bortezomib by inhibition of PRAS40-mediated protein synthesis

Fig. 6

PYCR1 interference increases bortezomib-mediated cell death in vitro. A OPM-2 and RPMI-8226 were cultured in a hypoxic environment for 72 h and treated with siRNA for PYCR1 and bortezomib. Apoptosis was measured using flow cytometry by staining for Annexin V and 7-AAD (n = 5). B-C OPM-2 and RPMI-8226 were cultured in a hypoxic environment for 72 h and treated with siRNA for PYCR1 and bortezomib. Apoptosis markers PARP and CASPASE 3 were measured on protein level by western blotting. Bar graphs represent the pixel intensity measured by Image Studio Lite v5.2. All graphs are shown relative to mock (n = 5). D-E OPM-2 and RPMI-8226 were cultured in a hypoxic environment for 72 h and treated with siRNA for PYCR1 and bortezomib. UPR markers IRE1a, p-SAPK/JNK and CHOP were measured on protein level by western blotting. Bar graphs represent the pixel intensity measured by Image Studio Lite v5.2. All graphs are shown relative to mock (n ≤ 6). All experiments were performed in hypoxic conditions. bz = bortezomib. Significance was determined by one-way ANOVA. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001

Back to article page