Fig. 3
TRPM7 silencing reduces the expression of glycolysis-related regulators, but enhances the OXPHOS-related regulators in ovarian cancer cells. A IHC analysis of TRPM7, HK2, PDK1, IDH3B and UQCRC1 expression in human ovarian cancer tissues and non-tumor ovarian tissues. Scale bar = 50 μm. B Correlation analysis between the levels of TRPM7 and HK2, PDK1, IDH3B, or UQCRC1 expression in ovarian cancer tissues. C-D Western blot and RT-qPCR analyses of HK2, PDK1, IDH3B and UQCRC1 expression in the indicated cells. E Immunofluorescent analysis of HK2, PDK1, IDH3B and UQCRC1 expression in ovarian cancer cells after stained with mouse anti-HK2, rabbit anti-PDK1, mouse anti-IDH3B, rabbit anti-UQCRC1 and subsequent Alexa Fluor™488-goat anti-mouse IgG and Alexa Fluor™ 594-goat anti-rabbit IgG as well as DAPI, scale bar = 50 μm. F Western blot analysis of PKM2 in the nuclear and cytoplasmic fractions following TRPM7 silencing in SKOV3 and HO8910 cells. G IHC analysis of HK2, PDK1, IDH3B and UQCRC1 expression (scale bar = 50 μm)