Fig. 7

TRPM7 silencing activates the AMPK signaling to promote ubiquitination and degradation of HIF-1α in ovarian cancer cells. A Western blot displayed that TRPM7 silencing decreased HIF-1 protein levels in ovarian cancer cells under a hypoxic condition. B In vivo ubiquitination assays indicated that the decreased HIF-1α protein by TRPM7 silencing was abrogated by treatment with MG132, suggesting that TRPM7 silencing promoted HIF-1α ubiquitination and degradation in SKOV3 cells. C There was no detectable HIF-1α protein in the SKOV3 cells under a normoxic condition. D Inhibition of AMPK activation by CC increased HIF-1α protein levels in both control and TRPM7 silencing SKOV3 cells under a hypoxic condition. E, F In vivo ubiquitination assays revealed that treatment with CC to inhibit AMPK activation mitigated the TRPM7 silencing-induced HIF-1α ubiquitination and degradation in ovarian cancer cells under both normoxic and hypoxic conditions