Fig. 1

FTO is downregulated and the total m⁶A level was increased in PTC. a-b Expression level of m⁶A modification enzymes in TCGA and GEO database (GSE60542, GSE27155 and GSE3467). c Relative expression levels of m⁶A modification enzymes in 30 pairs PTC tissues and paired non-cancerous tissues as determined by qRT-PCR. d Total m⁶A levels in 5 pairs PTC samples as determined by m⁶A RNA Methylation Quantification Kit. e Relative expression levels of FTO in 100 pairs of PTC tissues and adjacent non-cancerous tissues as determined by qRT-PCR. f Representative photographs of IHC staining for FTO protein expression in PTC tissues and adjacent non-cancerous tissues (Cohort 2). Scale bars: 50 μm (400X). g Representative photographs of western blotting for FTO protein expression in PTC tissues (T) and paired non-cancerous tissues (N) (Cohort 3). *P < 0.05, **P < 0.01