Fig. 3

FTO inhibition of PTC growth by modulating glycolytic metabolism in vitro and in vivo. a Extra cellular acidification rate (ECAR) as determined by Seahorse metabolic analysis after transfection with si-NC and si-FTO in K1 cells. b-c Glucose uptake (b) and Lactate production (c) were determined after transfected with si-NC and si-FTO in K1 cells. d mRNA expression level of glycolytic enzymes was determined by qRT-PCR after FTO knockdown. e Protein expression level of GLUT1, HK-II and LDHA were determined by western blotting after FTO knockdown in K1 cells. f-g CCK-8 assay (f) and colony formation assay (g) showing proliferation ability after transfection with si-NC or si-FTO and simultaneous treatment with or without 2-Deoxyglucose (2-DG) in K1 cells. h Representative images of ¹⁸F-FDG uptake by micro-PET imaging in sh-NC and sh-FTO xenograft mouse models. Tumor glucose uptake is marked by red circles and maximum uptake values (SUVmax) for xenografts determined by FDG-PET are shown. i ECAR as determined by Seahorse metabolic analysis after FTO overexpression in TPC1 cells. j-k Glucose uptake (j) and Lactate production (k) were determined after FTO overexpression in TPC1 cells. l GLUT1, HK-II and LDHA protein expression levels as determined by western blotting after FTO overexpression in TPC1 cells. m Representative images of ¹⁸F-FDG uptake by micro-PET imaging in FTO overexpression xenograft mouse models. Tumor glucose uptake is indicated by red circles and maximum uptake values (SUVmax) for xenografts determined by FDG-PET are shown. *P < 0.05, **P < 0.01