Fig. 1

Circulating tumor cell (CTC) enrichment and gene expression profile (GEP) analysis in MDA-MB-231 xenograft models. A Gel-like image of a capillary electrophoresis separation of MET amplicon following MDA-MB-231 cell spike-in and immuno-capture experiments. B Logarithmic curve of mean±standard deviation threshold cycles obtained by quantitative PCR for human ACTB as a function of MDA-MB-231 cell numbers (n=3 experimental replicates per point). C Dot plot of CTC numbers per blood volume in MDA-MB-231 xenograft models (n=3 experiments). D Heat map of pair-wise correlations using raw GEP data from technical duplicates (“a” and “b”) of human (“H”) and murine (“M”) universal reference RNA inputs mixed at different ratios (numbers indicate the RNA percentage)