Fig. 3

FADS3 regulates MDA-MB-231 cell motility, hematogenous dissemination and lung colonization ability. A Box and whiskers plot of FADS3 expression data (log₂(signal intensities)) using data from gene expression profile experiments GEP1 and GEP2. B (left) Dot plot of the pyrenedecanoic acid (PDA) excimer-to-monomer ratio in a fluorimetric membrane fluidity test, and (right) dot plot of the fraction of PDA excimer positive cells with representative images of fluorescence signals from PDA excimers incorporated at cell membrane level (40x objective). C Line chart representation of the mean±standard deviation (SD) optical density measured in a time-course proliferation colorimetric assay (n=3 observations in 3 lentiviral infection replicates). D (left) Dot plot of the number of migrating cells in a Boyden chamber assay, and (right) representative images of GFP expressing cells at the chamber bottom side (4x objective). E (left) Dot plot of the number of Matrigel invading cells (mean of 3 wells) in a Boyden chamber assay (n=3 independent experiments with 3 lentiviral infection replicates), and (right) representative images of GFP expressing cells at the chamber bottom side (4x objective). F (left) Dot plot of the number of vascular loops, and (right) representative images of GFP expressing cell loops (4x objective). G Line chart representation of the mean±SD sum of the tumor masses measured upon cell injection at the inguinal left and axillary right mammary fat pads of NOD SCID mice (n=6 shCTRL and n=7 shFADS3). H (left) Dot plot of single CTC (sCTC) counts, and (right) representative images of COX IV positive CTCs (60x oil immersion objective). I (left) Dot plot of CTC cluster (cCTC) count and representative images of (top right) COX IV positive cCTC and (bottom right) a COX IV negative leukocyte cluster (60x oil immersion objective). J (left) Dot plot of metastatic foci extent measured in lung sections, and (right) representative images of COX IV positive metastatic cell foci (10x objective)