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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Elevated expression of the rhythm gene NFIL3 promotes the progression of TNBC by activating NF-κB signaling through suppression of NFKBIA transcription

Fig. 1

Decreased NFIL3 mRNA indicates elevated NFIL3 protein in TNBC. A. The expression of six rhythm genes is significantly changed in breast cancer compared with normal breast tissues based on the GEPIA database. B. The mRNA expression of six rhythm genes was compared using qRT–PCR in 20 normal breast tissues and 20 breast cancer tissues (*p < 0.05, ns means there was no significant difference). C. The mRNA expression of EGR3 and NFIL3 was compared in different subtypes of breast cancer by using qRT–PCR (*p < 0.05, **p < 0.01, ***p < 0.001, ns means there was no significant difference). D. The expression of NFIL3 protein in different subtypes of breast cancer was evaluated using IHC (magnification 400×, scale bars = 50 μm, **p < 0.01). E. High NFIL3 protein levels predict poor prognosis in TNBC patients. F. The binding of NFIL3 in the promoter region of the NFIL3 gene was validated in Hs578T cells using a ChIP assay. G. A dual luciferase reporter assay was performed to test the suppressive effect of NFIL3 on the transcription of luciferase driven by the NFIL3 gene promoter in Hs578T cells (**p < 0.01). This experiment was repeated in triplicate. H. The overexpression of NFIL3 protein in two TNBC cell lines was verified using western blot after forced expression of exogenous NFIL3. The relative expression of NFIL3 to GAPDH was normalized using Image J software (**p < 0.01). I. Relative NFIL3 pre-mRNA levels were detected by qRT–PCR after overexpressing exogenous NFIL3 (*p < 0.05, **p < 0.01)

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