Fig. 5

NFKBIA suppresses the activity of NF-κB signaling and is correlated with the infiltration of immune cells in breast cancer. A. The impact of NFKBIA overexpression or knockdown on the translocation of NF-κB from the cytoplasm to the nucleus in Hs578T cells detected by immunofluorescence assay. B. A luciferase reporter assay was used to detect the activity of NF-κB signaling in Hs578T cells upon NFKBIA overexpression or knockdown with or without TNF-α (25 ng/mL) stimulation (*p < 0.05, **p < 0.01, ***p < 0.001). The experiment was repeated in triplicate. C. The positive correlation between NFKBIA mRNA level and the infiltration of CD8+ T cells, activated CD4 memory T cells and M1 cells in the breast cancer was obtained by Pearson Correlation analysis based on the TCGA dataset (p < 0.05). D. The negative correlation between NFKBIA mRNA level and the infiltration of resting mast cells, eosinophils cells and M2 cells in the breast cancer was obtained by Pearson Correlation analysis based on the TCGA dataset (p < 0.05). E. The representative expression of NFKBIA, CD8, CD68, CD163 and CD16 at protein level was detected by IHC (magnification, 400×, scale bars = 50 μm). F. Pearson correlation analysis between the expression of NFKBIA protein and the level of infiltrated CD8+ T cells, M1 and M2 cells in 60 TNBC tissues (p < 0.01)