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Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: Increased Drp1 promotes autophagy and ESCC progression by mtDNA stress mediated cGAS-STING pathway

Fig. 1

Drp1-mediated mitochondrial fission promoted survival of ESCC cells in vitro and in vivo. (A) Representative immunohistochemical (IHC) staining images of Drp1 and TFAM in paired ESCC tissues (n = 61). Scale bar: 100 μm. (B and C) Western blot and qRT-PCR analyses for the expression levels of Drp1 TFAM in paired tissues from ESCC patients. T, tumor; P, peritumor. The relative expression ratio of tumor to peritumor was log2-transformed for further analysis. (D)Kaplan–Meier curve analysis of overall survival in ESCC patients grouped by the expression of Drp1 in tumor tissues. Patients were grouped into high or low level using the median value of IHC scores. Death/total number of patients in each subgroup were presented. (E)Colony-forming potential were detected in ESCC cells with treatment as indicated. Scale bar: 10 mm. The data shown are the mean ± SEM from three separate experiments. (F) Cell proliferation was evaluated by EdU incorporation assay in KYSE-30 cells as indicated. Scale bar: 200 μm. (G) Cell viability of KYSE-30 ESCC cells with Drp1 overexpression were evaluated using the MTS cell proliferation assay. (H and I) Tumor growth curves and weight of subcutaneous xenograft tumor model developed from ESCC cells with Drp1 overexpression (n = 6). The tumor volumes were calculated according to the formula (L × W2)/2 and presented as mean ± SEM. (J) Representative immunohistochemical (IHC) staining images of Ki67 in xenograft tumors developed from KYSE-30 cells with Drp1 overexpression. Scale bar: 100 μm. The data shown are the mean ± SEM from three separate experiments. Drp1, expression vector encoding Drp1; EV, empty vector. P value from t tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001

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