Skip to main content
Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Increased Drp1 promotes autophagy and ESCC progression by mtDNA stress mediated cGAS-STING pathway

Fig. 4

cGAS-STING signaling pathway is involved in Drp1 overexpression-mediated autophagy. (A) Heat map analysis of gene expression in ESCC cells as indicated. (B, C and D) Western blot analyses were performed in ESCC cells as indicated with antibodies specific for the proteins shown. (E) Representative transmission electron microscopy images of double-membrane autophagosomes in ESCC cells as indicated. Scale bar: 0.5 μm. (F) Representative images of fluorescent LC3B puncta (green) in ESCC cells as indicated. Scale bar: 10 μm. The data shown are the mean ± SEM from three separate experiments. Drp1, expression vector encoding Drp1; EV, empty vector; NC, negative control; CQ, chloroquine; siSTING, siRNAs against STING; sicGAS, siRNAs against cGAS. P value from t tests. **, P < 0.01; ***, P < 0.001

Back to article page