Skip to main content
Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Increased Drp1 promotes autophagy and ESCC progression by mtDNA stress mediated cGAS-STING pathway

Fig. 7

Cytosolic mtDNA stress mediates the cGAS-STING signaling activation, autophagy induction, and cell proliferation of ESCC. (A) Western blot analyses were performed in ESCC cells as indicated with antibodies specific for the proteins shown.(B) Representative transmission electron microscopy images of double-membrane autophagosomes in ESCC cells as indicated. Scale bar: 0.5 μm. (C) Representative images of fluorescent LC3B puncta (green) in ESCC cells as indicated. Scale bar: 10 μm. (D) Colony-forming potential were detected in KYSE-30 cells as indicated. Scale bar: 10 mm. (E) Cell proliferation was evaluated by EdU incorporation assay in KYSE-30 cells as indicated. Scale bar: 200 μm. The data shown are the mean ± SEM from three separate experiments. Drp1, expression vector encoding Drp1; EV, empty vector; NC, negative control; HI DNase I, heat inactivated DNase I. P value from t tests. *, P < 0.05; **, P < 0.01; ***, P < 0.001

Back to article page