Fig. 1

High HDAC7 expression was correlated with poor NSCLC prognosis, and HDAC7 overexpression promoted NSCLC proliferation. a Kaplan–Meier survival analysis of HDAC7 expression from the Kaplan–Meier plotter database. b Western blots of HDAC7 expression in tumor (T) and paired adjacent nontumor (N) tissues from 12 NSCLC patients. c Representative HDAC7 IHC images of NSCLC and adjacent non-tumor tissues. Scale bar, 200 μm and 20 μm (inset), respectively. IHC analysis (d) and Kaplan–Meier survival analysis (e) of HDAC7 expression in 319 NSCLC patients. f The growth curve of indicated NSCLC cells. g Representative images and analysis of EdU incorporation assay. EdU-positive cells (red) and Hoechst 33342–stained cells (blue). Scale bar, 100 μm (inset). h Representative images and analysis of colony formation assay. i Representative western blotting of HDAC7, CDK2 and Cyclin A2 in indicated NSCLC cells. j Representative western blotting of whole cell lysates (WCL) at indicated times. A549 cells were synchronized in G0/G1 by serum starvation followed by serum re-addition. k Gross photograph of subcutaneous xenograft tumors, tumor growth curve, changes of nude mice body weight, and subcutaneous tumor weight in each group. l Representative western blotting of HDAC7, CDK2, and Cyclin A2 in subcutaneous tumor tissues. All the data are expressed a mean ± SD. *P < 0.05. LV, lentivirus