Fig. 2

HDAC7 overexpression facilitated NSCLC metastasis in vitro and vivo. a Representative scratch wound assay images and results. Migration ability is expressed as the mean scratch area at each time point. The initial scratched area (0 h) was set as 100%. b Representative transwell migration assay images and results. Representative western blotting of HDAC7, E-cadherin, and Snail in HDAC7 overexpression and knockdown cells (c) and xenograft tumor tissues (d). Representative fluorescence images (e) and fluorescence signals analysis (f) of pulmonary metastases 4 weeks after tail vein injection. g Representative HE staining images of lung samples from indicated groups. Scale bar, 1 mm and 100 μm (inset), respectively. h The number of metastatic nodules per lung in HE staining images. i Representative general morphology of surface lung metastases. The black triangular arrows indicate the metastatic nodules. β-actin was used as internal control. All the data are expressed as mean ± SD. *P < 0.05. LV, lentivirus