Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: HDAC7 promotes NSCLC proliferation and metastasis via stabilization by deubiquitinase USP10 and activation of β-catenin-FGF18 pathway

Fig. 3

HDAC7 regulated cell proliferation and metastasis via FGF18 in NSCLC. a The volcano plot of DEGs identified from RNA-seq data of SK-LU-1 cells overexpressing HDAC7 or the control. b Representative western blotting of FGF18 and FGFR3 in HDAC7 overexpression and knockdown cells. c Representative western blotting of whole cell lysates (WCL) at indicated times. d Representative western blotting of HDAC7, FGF18, CDK2, and Cyclin A2 in the indicated groups. e Representative images and results of colony formation assay. f Gross photograph of subcutaneous xenograft tumors, tumor growth curve and tumor weight in indicated groups. g Representative scratch wound assay images and results. h Representative western blotting of E-cadherin, Snail, and vimentin in indicated groups. i Representative fluorescence images and data analysis of pulmonary metastases 4 weeks after tail vein injection. j The general morphology of surface lung metastases. The black triangular arrows indicate the metastatic nodules. The number of metastatic nodules per lung in HE images (k) and representative HE staining images (l) from lung samples of indicated groups. Scale bar, 1 mm and 100 μm (inset), respectively. β-actin was used as internal control. All the data are expressed as mean ± SD. *P < 0.05. LV, lentivirus

Back to article page